ISO INTERNATIONAL STANDARD 15753 Second edition 2016-04-01 Animal and vegetable fats and oils - Determination of polycyclic aromatic hydrocarbons Corps gras d'origines animale et vegetale - Détermination des hydrocarbures aromatiques polycycliques Reference number ISO 15753:2016(E) ISO International Organization for Standardization =ZHEJIANG INST OF STANDARDIZATION C1 5956617 @IS02016 vided by IHS under Not for Resale, 2016/6/2 08:59:20 ted without license from IHS IS0 15753:2016(E) COPYRIGHTPROTECTEDDOCUMENT IS0 2016, Published in Switzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyrightoffice Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel. +41 22 749 01 11 Fax +41 22 749 09 47 [email protected] www.iso.org Internatinair Prganization for Standardization ensee-ZHEJIANG INST OF STANDARDIzARoISQ16- All rights reserved etworking permitted without license from IHS IS0 15753:2016(E) Contents Page Foreword ..iv 1 Scope 2 Normative references .1 3 Terms and definitions ..1 4 Principle ..2 5 Reagents and materials ..2 6 3 Apparatus. 7 Sampling. ..4 8 Sample preparation .5 9 Procedure for determination of PAHs from fats and oils: General method .5 9.1 Preliminary remarks .5 9.2 Blank. .5 9.3 Determination of recovery values (without matrix) .5 9.4 Extraction (liquid/liquid extraction). .5 9.5 Purification on Ci8-bonded phase cartridge (solid/liquid extraction) .6 9.6 Purification on Florisil-bonded phase cartridge (solid/liquid extraction) .7 10 Procedure for determination of PAHs from fats and oils: Method specific for coconut oil....8 10.1 First extraction (liquid/liquid extraction) 8 10.2 Second extraction (liquid/liquid extraction) .8 10.3 Purification on C18-bonded phase cartridge (solid/liquid extraction) ..8 10.4 Purification on Florisil-bonded phase cartridge (solid/liquid extraction) ..9 11 High-performance liquid chromatography (HPLC) ..10 11.1 Operating conditions. ..10 11.2 Detection parameters .10 11.3 Analysis of samples and standards .12 11.4 Confirmation of the presence of PAHs ..12 12 Expression of results .12 13 Precision .13 13.1 Interlaboratory test. .13 13.2 Repeatability .13 13.3 Reproducibility ..13 14 Test report .13 Annex A (informative) Recovery values, flow charts, chromatograms and injection sequences S....15. Annex B (informative) Results of the interlaboratory test ..20 Bibliography .23 iii HEJIANG INST OF STANDARDIZATION C1 5956617