ISO INTERNATIONAL STANDARD 16000-20 First edition 2014-12-01 Indoor air Part 20: Detection and enumeration of moulds - Determination of total spore count Airintérieur- Partie 20: Détection et denombrement des moisissures - Détermination du nombre total de spores Reference number IS0 16000-20:2014(E) SO International Organization for Standardization Institute of Sta andardization5956617 IS02014 vided by IHS under I ted without license from IHS IS0 16000-20:2014(E) COPYRIGHTPROTECTEDDOCUMENT @ IS0 2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISOcopyrightoffice Case postale 56:CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland Prganizationfor Standardization Not for Resale, 2015/3/20 05:40:33 networking permitted without license from IHS IS0 16000-20:2014(E) Contents Page Foreword ..iv Introduction. ...vi 1 Scope. 2 Terms and definitions 3 Principle of method ..2 4 .2 Apparatus and materials 5 Reagents .3 5.1 General .3 5.2 Lactophenol blue solution .3 6 Measurement procedure .3 6.1 Sampling. .3 6.2 Direct microscopy. .4 6.3 Calculation and expression of results .5 6.4 Transport and storage .6 7 Quality assurance .6 8 Calibration of flow rate, function control, and maintenance of the sampling system .6 9 Sampling protocol .6 10 Performance characteristics 7 Annex A (informative) Examples of impactors ..11 Annex B (normative) Sample exchange for method validation .12 Bibliography ..13 iii vided by IHS under thout license from IHS