ISO INTERNATIONAL STANDARD 16187 First edition 2013-08-01 Footwear and footwear components Test method to assess antibacterial activity Chaussure et composants de chaussure - Méthode d'essai pour évaluerI'activité antibactérienne Reference number ISO 16187:2013(E) ISO @IS02013 No reproduction or networking permitted without license from IHS Not for Resale IS0 16187:2013(E) COPYRIGHTPROTECTEDDOCUMENT @ISO2013 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Case postale 56 : CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax + 41 22 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ IS0 2013 - All rights reserved or networking permited without license from IHS Not for Resale IS0 16187:2013(E) Contents Page Foreword ..iv 1 Scope 2 Normative references ..1 3 Terms and definitions ..1 4 Safety 5 Apparatus and materials. ..2 6 Reagents and culture medium .2 7 Test microorganisms ..4 7.1 Test strains ..4 7.2 Storage of strains .4 8 Preparation of test inoculums ..4 9 Preparation of test samples ..4 9.1 General, ..4 9.2 Test specimen ..5 9.3 Pre-treatment of the test specimen .5 10 Test procedure. ..5 11 Expression of results ..5 12 Test report Annex A (normative) Static challenge test Annex B (normative) Film contact method. Annex C (normative) Dynamic challenge test ..12 Annex D (informative) Summarized results of round robin tests ..14 Bibliography ..16 iii without license from IHS Not for Resale