ISO INTERNATIONAL STANDARD 16308 First edition 2014-09-15 Water quality Determination of glyphosate and AMPA Method using high performance liquid chromatography (HPLC) with tandem mass spectrometric detection Qualité de I'eau - Détermination du glyphosate et de I'AMPA - Méthode par chromatographie en phase liquide a haute performance (CLHP) avec détection par spectrométrie de masse en tandem Reference number IS0 16308:2014(E) ISO @IS02014 ed without license from IHS Not for Resale IS0 16308:2014(E) COPYRIGHT PROTECTED DOCUMENT @ISO2014 All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of therequester. iso copyright office Case postale 56 CH-1211 Geneva 20 Tel. + 41 22 749 01 11 Fax +4122 749 09 47 E-mail [email protected] Web www.iso.org Published in Switzerland @ IS0 2014 - All rights reserved Not for Resale IS0 16308:2014(E) Contents Page Foreword ..iv Introduction. ..V 1 Scope. ..1 2 Normative references ..1 3 Principle 4 Interferences ..2 5 Reagents .2 6 Apparatus ..4 7 Sampling. .5 8 Procedure. .5 8.1 Pre-treatment (Suspended particular matter) .5 8.2 Chelate break and derivatization .5 8.3 Pre-concentration. .6 8.4 Chromatographic determination .7 8.5 Identification and confirmation of the analytes .8 8.6 Blank control monitoring .8 9 Calibration .8 9.1 Concentration ranges .8 9.2 Matrix-matched calibration ..8 9.3 Internal standard calibration ..9 10 Expression of results ..10 11 Test report ..10 Annex A (informative) Performance data ..11 Annex B (informative) Examples of chromatographic conditions .14 Annex C (informative) Examples of chromatograms ..15 Annex D (informative) Analysis of gluphosinate ..16 Annex E (informative) Pre-treatment of hard water samples ..20 Bibliography .22 ii e with I: without license from IHS Not for Resale