ISO INTERNATIONAL STANDARD 13304-2 First edition 2020-07 Radiological protection Minimum criteria for electron paramagnetic resonance (EPR) spectroscopy for retrospective dosimetry of ionizing radiation - Part 2: Ex vivo human tooth enamel dosimetry Radioprotection-Criteresminimauxpourlaspectroscopiepar resonanceparamagnetiqueelectronique(RPE)pour ladosimétrie retrospectivedesrayonnementsionisants Partie2:Dosimétrie exvivo a partirdeI'émail dentaire humain Reference number IS0 13304-2:2020(E) ISO @ ISO 2020 IS013304-2:2020(E) COPYRIGHTPROTECTEDDOCUMENT ISO2020 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may below or ISo's member body in the country of the requester. ISO copyright office CP 40i : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone: +41 22 749 01 11 Email: [email protected] Website: www.iso.org Published in Switzerland ii IS02020-Allrights reserved IS013304-2:2020(E) Contents Page Foreword V Introduction .vi 1 Scope. .1 2 Normativereferences .1 3 Terms and definitions .1 4 Apparatus. .5 4.1 SpecificationsforEPR spectrometer. 5 4.2 Spectrometer sensitivity 5 4.3 Microwavebridge 5 4.4 Magnetic field 5 4.5 Microwaveresonator 6 5 Preparation of tooth enamel samples 6 5.1 General 6 5.2 Applicablegrain size 7 6 Measurement of the EPR spectrum 7 6.1 Description of spectrum 7 6.2 Applicablemeasurementparameters and conditions 8 6.2.1 General. 8 6.2.2 Microwave power. 8 6.2.3 Magnetic centre field 8 6.2.4 Magnetic field sweep width 8 6.2.5 Magnetic field sweep time. 8 6.2.6 Time constant of signal channel receiver 6 6.2.7 EPR spectrum resolution. 9 6.2.8 Conversion time of spectrumacquisition 9 6.2.9 Magnetic field modulationamplitude 9 6.2.10 Number of spectrum accumulations 9 6.2.11 Samplepositioningand loading .10 6.2.12 DependenceofEPRsignalintensityonsamplemass .10 6.2.13 Use of standard samples. 10 6.2.14 Number ofmeasurement repetitions .11 7 Assessment of the RIS intensity .11 7.1 General .11 7.2 IntrinsicEPRsignalsfrommicrowaveresonatorandsampletube ..12 8 Irradiationoftoothenamel calibrationsamplesforlowlinearenergytransfer (LET) exposure .12 9 Conversion of the Ris intensity intoan estimate of absorbed dose .13 10 Calculationofuncertaintyondoseestimate .14 11 Minimum detectable dose .15 12 Confidentialityandethicalconsiderations. .16 13 Laboratory safety requirements .16 13.1 General. .16 13.2 Magnetic field safety requirements .16 13.3 Electromagnetic frequency requirements 17 13.4 Chemical safetyrequirements 17 13.5 Health risks from tooth samples. .17 .17 13.6 Optical safety requirements 14 Responsibility of the customer .17 @ IS0 2020 - All rights reserved iii