ISO INTERNATIONAL STANDARD 13794 Second edition 2019-10 Ambient air Determination of asbestos fibres Indirect-transfer transmission electron microscopy method Airambiant-Dosagedesfibresd'amiante-Méthodepar microscopieélectronique atransmissionpartransfert indirect Referencenumber IS0 13794:2019(E) ISO @ IS0 2019 IS0 13794:2019(E) COPYRIGHTPROTECTEDDOCUMENT IS02019 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting below or Iso's member body in the country of the requester. ISO copyright office CP 401 : Ch. de Blandonnet 8 CH-1214 Vernier, Geneva Phone:+4122749 0111 Fax: +41 22 749 09 47 Email: [email protected] Website: www.iso.org Published in Switzerland ii @ IS0 2019 - All rights reserved IS0 13794:2019(E) Contents Page Foreword ..vi Introduction. ..vi 1 Scope. 2 Normative references 3 Terms and definitions 4 Symbols and abbreviated terms 5 Type of sample ..8 6 Range ..8 7 Limit of detection. ..8 8 Principle. ..8 9 Reagents .9 10 Apparatus. .10 10.1 Air sampling. ..10 10.1.1 Filter cassette ..10 10.1.2 Sampling pump .10 10.1.3 Stand. .10 10.1.4 Personal sampling ..10 10.1.5 Flowmeter .10 10.2 Specimenpreparation laboratory .11 10.3 Equipment for analysis. .11 10.3.1 Transmission electron microscope .11 .13 10.3.2 Energy dispersive X-ray analyser 10.3.3 Plasma asher .13 10.3.4 Vacuum coating unit. ..13 10.3.5 Sputter coater .13 10.3.6 .13 Beakers. 10.3.7 Vacuum source. .13 10.3.8 Glass filtration apparatus ..14 10.3.9 Solvent washer (Jaffe washer) .14 10.3.10 Condensation washer .15 .16 10.3.11 Slide warmer or oven. 10.3.12Ultrasonic bath .16 10.3.13 Carbon grating replica. ..16 10.3.14Calibration specimengridsforEDXA ..16 10.3.15 Carbon rod sharpener ..17 10.3.16Disposabletipmicropipettes .17 10.3.17Thermometer ..17 10.3.18 Stopwatch. .17 10.4 Consumable supplies. ..17 10.4.1 Copper or nickel electron microscopegrids .17 10.4.2 Gold or nickelelectron microscopegrids. .17 10.4.3 Aluminium foil .17 10.4.4 Carbon rod electrodes .17 10.4.5 Routineelectronmicroscopytoolsand supplies. ..18 10.4.6 Reference asbestos samples. .18 10.4.7 Reference samples of mineral fibres other than asbestos ..18 11 Airsamplecollection ..18 11.1 Calculation of analytical sensitivity .18 11.2 Sample collectionprocedure .19 @ IS0 2019 - All rights reserved iii