ISO INTERNATIONAL STANDARD 13885-1 Third edition 2020-07 Gel permeation chromatography (GPC) 一 Part 1: Tetrahydrofuran (THF) as eluent Chromatographie par perméation de gel (GPC) - Partie 1: Utilisation de tétrahydrofurane (THF) comme éluant Reference number IS0 13885-1:2020(E) ISO @ ISO 2020 IS013885-1:2020(E) COPYRIGHTPROTECTEDDOCUMENT IS02020 All rights reserved. Unless otherwise specified, or required in the context of its implementation, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting below or Iso's member body in the country of the requester. ISo copyright office CP 40i: Ch.de Blandonnet 8 CH-1214 Vernier,Geneva Phone: +4122749 0111 Email: [email protected] Website: www.iso.org Published in Switzerland i @ IS0 2020 - All rights reserved IS0 13885-1:2020(E) Contents Page Foreword V 1 Scope. 1 2 Normative references .1 3 Terms and definitions .1 4 Principle .2 5 Apparatus. .2 5.1 Eluent supply 2 5.2 Pump. 3 5.3 Injection system. 3 5.4 Separation columns 3 5.5 Column temperature control 5 5.6 Detector. 5 6 Reagents. .5 7 Calibration of the apparatus 6 7.1 General 6 7.2 Requirements for the calibration standards 6 .7 7.3 Preparation of the calibration solutions for injection 7.4 Conditions for calibration runs. .7 7.5 Measurementof elutionvolume .7 7.6 Plotting the calibration curve .7 8 Sampling. 9 Preparation forthetest .8 9.1 Preparation of the injection solution. .8 9.2 Preparation of the apparatus. .9 10 Analytical parameters. 11 Data acquisition and evaluation. .9 11.1 General. 9 11.2 Calculationofthenetchromatogramfromtherawdata 10 11.2.1 Determination ofthebaseline 10 11.2.2 Correctionofthemeasuredvaluesandofthenetchromatogram 10 11.2.3 Evaluation limits. 10 11.3 Calculation of the average values. 10 11.4 Calculation of the distribution curves. 11 12 Precision .12 12.1 General 12 .12 12.2 Repeatability 12.3 Reproducibility .12 13 Test report. .13 13.1 General 13 13.2 General data on the equipment and settings 13 13.2.1 Data on the equipment used. 13 13.2.2 Calibration 13 13.2.3 Evaluation .14 13.3 Special data on the sample .14 AnnexA (informative) Conversion ofexperimentalparameters for variant column sizes. .16 Annex B (informative) Example ofa data sheet for a polymer standard ..17 AnnexC (informative)Explanations. .18 iii IS0 2020-All rights reserved