ISO INTERNATIONAL 13885-2 STANDARD First edition 2020-07 Gel permeation chromatography (GPC) Part 2: N,N-Dimenthylacetamide (DMAC)as eluent Chromatographie par permeation degel (GPC)- Partie 2:Eluant au N,N-Dimethylacetamide (DMAC) Referencenumber ISO 13885-2:2020(E) @ISO 2020 ISO 13885-2:2020(E) COPYRIGHT PROTECTED DOCUMENT OISO2020 All rights reserved.Unless otherwise specified,or required in the context of its implementation,no part ofthis publication may be reproduced or utilized otherwise in any form or by any means,electronic or mechanical,including photocopying,or posting on the internet or an intranet,without prior written permission.Permission can be requested from either IsO at the address below or ISO's member body in the country ofthe requester. ISO copyright office CP 401:Ch.de Blandonnet 8 CH-1214 Vernier,Geneva Phone:+41227490111 Email:[email protected] Website:wwwiso.org Published in Switzerland ii O ISO 2020-All rights reserved ISO 13885-2:2020(E) Contents Page Foreword 1 Scope 2 Normative references 3 Terms and definitions 4 Principle 5 Apparatus 5.1 Eluent supply 5.2 Pump 5.3 Injection system 5.4 Separation columns 5.5 Column temperature control 5.6 Detector Reagents 6 7 Calibration of the apparatus 7.1 General 7.2 Specification forthe calibration standards 7.3 Preparation ofthe calibration solutions for injection 7.4 Conditions for calibration runs 7.5 Measurementofelution volume 7.6 Plotting the calibration curve 8 Sampling 9 Preparation for the test 9.1 Preparation ofthe injection solution 9.2 Preparation ofthe apparatus 10 Analytical parameters 11 Data acquisitionand evaluation 10 11.1 General 11.2 Calculation of the net chromatogram from the raw data 10 11.2.1 Determinationof the baseline 10 11.2.2 Correction ofthemeasured values and of thenet chromatogram 10 11.2.3 Evaluation limits 10 11.3 Calculation of the average values 11.4 Calculation of the distribution curves 12 12 Precision 12.1 General 2 12.2 Repeatability 12 12.3 Reproducibility 13 13 Test report 13.1 General 13 13.2 General data on the equipment and settings 13 13.2.1 Data on the equipment used 13 13.2.2 Calibration 14 13.2.3 Evaluation 14 13.3 Special data on the sample for variant column sizes AnnexA(informative)Conversionofexperimental parameters ....16 Annex B (informative)Example of a data sheet for apolymer standard AnnexC(informative)Explanations 19 OISO 2020-Allrights reserved ili