ISO INTERNATIONAL STANDARD 9555-4 First edition 1992-11-01 Measurement of liquid flow inopen channels Tracer dilution methods for the measurement of steadyflow Part 4: Fluorescenttracers Mesurededebitdes liquidesdans les canaux decouverts-Methodes de dilutionen regimepermanent utilisant destraceurs- Partie 4:Traceurs fluorescents Referencenumber iSO 9555-4:1992(E) ISO 9555-4:1992(E) Contents Page 1 Scope 2 Normative reference Definitions 3 4 Tracers used 4.1 General 4.2 Fluorescein 4.3 Rhodamine B 2 4.4 Acid yellow 7 2 4.5 Sulfo rhodamine B 2 4.6 Pyranine 2 4.7 Rhodamine WT 2 5 Tracermeasurement 2 5.1 Principles 5.2 Field measurement 5 5.3 Laboratory measurements 6 Environmentalfactorsaffectingtracers 6 6.1 Chemical factors 6 6.2 Physical factors 6 6.3 Biological factors 7 Techniquesfortracerinjection Sampling techniques 8 Analysis and computations 8 10 Specialrequirements All rights reserved. No part of this publication may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing frorn the publisher. InternationalOrganizationforStandardization CasePostale56·CH-1211Geneve20·Switzerland Printed in Switzerland ii ISO 9555-4:1992(E) Annex A General characteristics of commonly used fluorescent tracers 9 ili