ICS 11.220 B 41 团体标准 T/CVMA 21—2020 动物源性细菌药物敏感性试验 肉汤稀释法 Drug sensitivity test for animal -derived bacteria - broth dilution method 2020-12-22发布 2020-12-22实施 中 国 兽 医 协 会 发布 中国兽医协会 CVMA全国团体标准信息平台 T/CVMA 21 -2020 I 目 次 前言 ................................ ................................ ................................ ................................ ......... III 动物源性细菌药物敏感性试验 肉汤稀释法 ................................ ................................ ......... 1 1 范围 ................................ ................................ ................................ ................................ ....... 1 2 规范性引用文件 ................................ ................................ ................................ ................... 1 3 术语和定义、符号和缩略语 ................................ ................................ ............................... 1 4 试剂与材料 ................................ ................................ ................................ ........................... 2 5 器械与耗材 ................................ ................................ ................................ ........................... 2 6 培养基的确 定 ................................ ................................ ................................ ....................... 2 7 抗菌药物的确定 ................................ ................................ ................................ ................... 3 8 菌株确定 ................................ ................................ ................................ ............................... 3 8.1 质控菌株 ................................ ................................ ................................ ...................... 3 8.2 病原菌株 ................................ ................................ ................................ ...................... 3 8.2.1 病原菌株选择的要求 ................................ ................................ ............................. 3 8.2.2 病原菌株选择的操作要求 ................................ ................................ ..................... 3 9 药物敏感性试验 ................................ ................................ ................................ ................... 3 9.1 宏量肉汤稀释法（试管法 ) ................................ ................................ ........................ 3 9.1.1抗菌药物的制备和储存稀释 ................................ ................................ .................. 3 9.1.2 待测菌株的制备和接种 ................................ ................................ ......................... 4 9.2 微量肉汤稀释法 ................................ ................................ ................................ .......... 4 9.2.1 抗菌药物的制备和储存稀释 ................................ ................................ ................. 4 9.2.2 待测菌株的制备和接种 ................................ ................................ ......................... 4 10 培养方法 ................................ ................................ ................................ ............................. 4 11 结果判定 ................................ ................................ ................................ ............................. 4 附录 A（规范性） ................................ ................................ ................................ ................... 6 A.1 MHB 培养基 ................................ ................................ ................................ ............... 6 A.2 营养琼脂培养基 ................................ ................................ ................................ ......... 6 A.3 药物储备液 ................................ ................................ ................................ ................. 6 A.4 药物工作液 ................................ ................................ ................................ ................. 6 附录 B（规范性） ................................ ................................ ................................ ................... 7 药物敏